LOCUS VatM-gfp\/ 9251 bp DNA circular SYN 5-JUN-2000 DEFINITION VatM-gfp in cloning vector pDXA-3H. ACCESSION KEYWORDS SOURCE synthetic construct. ORGANISM synthetic construct artificial sequence. REFERENCE 1 (bases 1 to 6105) AUTHORS Clarke, M., Koehler, J., Arana, Q., Liu, T., Heuser, J., and Gerisch, G. TITLE Dynamics of the vacuolar H+-ATPase in the contractile vacuole complex and the endosomal pathway of Dictyostelium cells. JOURNAL J. Cell Sci. 115, in press. (2002). MEDLINE REFERENCE AUTHORS TITLE JOURNAL COMMENT This sequence has not been submitted to GenBank. NOTE: The end of the GFP to pDXA is not verified by sequencing COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|192623600| COMMENT VNTDBDATE|196789358| COMMENT VNTNAME|VatM-gfp / pDXA-3H| COMMENT VNTAUTHORNAME|Quyen Arana| COMMENT VNTAUTHORTEL|(405) 271-7660| COMMENT VNTAUTHORFAX|(405) 271-3153| COMMENT VNTAUTHOREML|aranaq@omrf.ouhsc.edu| COMMENT VNTAUTHORAD1|Molecular and Cell Biology| COMMENT VNTAUTHORAD2|825 NE13th Str| COMMENT VNTAUTHORAD3|Oklahoma City, OK 73104| COMMENT VNTOAUTHORNAME|UNKNOWN| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Animal/Other Eukaryotic FEATURES Location/Qualifiers terminator 1..270 /vntifkey="43" /label=Terminator_1\(CP1) rep_origin 5108..5684 /vntifkey="33" /label=Rep_Origin_1 /note="Ddp2 ori" promoter 5700..6027 /gene="actin 15" /vntifkey="29" /label=Promoter_P(actin\15)_1 gene 5700..6027 /gene="actin 15" /vntifkey="60" promoter complement(1581..2260) /vntifkey="30" /label=act6\pro /note="Actin6 Promoter" misc_feature complement(334..1580) /vntifkey="21" /label=Neo /note="Neomycin resistance gene" rep_origin 2450..2550 /vntifkey="33" /label=ori /note="Not an accurate placement!" misc_feature 3315..4353 /vntifkey="21" /label=amp /note="Not an accurate placement!" misc_feature 6046..6048 /vntifkey="21" /label=VatM\start misc_feature 8494..8523 /vntifkey="21" /label=[GA]5\linker misc_feature 6046..8487 /vntifkey="21" /label=VatM\gene misc_feature 8524..9240 /vntifkey="21" /label=GFP\[S65T]\gene BASE COUNT 2811 a 1844 c 1782 g 2813 t 1 others ORIGIN 1 aattcaagtt ttaccataaa ataaaaaaaa aaaataaaaa aaaacaaatc caattgtttt 61 tatattaaat aattaaaata aatgtaacta taactataaa tataataata tatacttata 121 tatactaact ctatttcttt tttttttttt tttttttaga tttaataaaa aattttattt 181 atttttaaat tctttttttt tttttttttt tttttttttt ttttctaaac taaatatttg 241 gtataaattt aaattatact agttgaaaca aagtttgata caccacatgt attctttcct 301 cttcttaaat aaatgtatcc ttgttctccc caactttttt ttttttttaa caaaattaat 361 tattagtaaa ctaaattatt atttttttta ttattattat tttaaaattt aaaatactta 421 ctctgcaccc caagaattga tcccaaagcg gccatcgtgc ctccccactc ctgcagttcg 481 ggggcatgga tgcgcggata gccgctgctg gtttcctgga tgccgacgga tttgcactgc 541 cggtagaact ccgcgaggtc gtccagcctc aggcagcagc tgaaccaact cgcgagggga 601 tcgagcccgg gccgggtggg cgaagaactc cagcatgaga tccccgcgct ggaggatcat 661 ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg cggcggtgga 721 atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga accccagagt 781 cccgctcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg 841 gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata 901 tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg 961 atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg 1021 gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa cagttcggct 1081 ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc 1141 cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga 1201 tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca 1261 aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc 1321 gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat 1381 agccgcgctg cctcgtcctg cagttcattc agggcaccgg acaggtcggt cttgacaaaa 1441 agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc 1501 tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc 1561 aatccatctt gttcaatcat gcgaaacgat ccagcttgaa catcttctcc gtccatttta 1621 tattatattt atttatttga ttattttttt gaattaatta aaaaaaaaaa aaaaatttca 1681 ttttataatc tcagaaacct caaaaaaaaa aaaataaaaa ataaaaaata taaaaaaata 1741 aaaataaaat cccaatttta aagcgaaaaa ccacccatgg tttgaaaatt tcaatcaatt 1801 tcaaataact ttacttaact tttaacttta agctggtcac ttggtttcct tgtttggaaa 1861 aaaaaaanta ccatgactaa actttaaatc tatttttttt ttataattat tatttttcat 1921 actctttttt ttttatataa aatatccatc attatcttca aaataaaaaa ataattacaa 1981 aaagtgaata ataattaaat aattaataaa cctttacact tttttttttc tttttacagt 2041 ttttatattt attttaattt tattattttg cgcaactttg aaaaaataaa aaagataaag 2101 tttttataaa tatctatcaa aaaagatatc gttgttgatt gattattttg gataattttt 2161 ctttaaattg gggcaaatct gtaattttca gattaataag atttttcctt tttatttttc 2221 tttttacttt ttgtaatttt taaaaataaa cctctgaatt aattcggcgt aatcatggtc 2281 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 2341 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 2401 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 2461 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 2521 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 2581 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 2641 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 2701 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 2761 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 2821 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc 2881 acgctgtagg tatctcagtt cggtgtaggt cgttcggtcc aagctgggct gtgtgcacga 2941 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 3001 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 3061 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 3121 gacactattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 3181 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 3241 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 3301 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 3361 cttcacctag atccttttaa attaaaatga agtatttaaa tcaatctaaa gtatatatga 3421 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 3481 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 3541 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 3601 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 3661 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 3721 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 3781 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 3841 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 3901 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 3961 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 4021 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 4081 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 4141 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 4201 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 4261 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 4321 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 4381 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga 4441 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct 4501 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac 4561 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt 4621 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca 4681 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcaatt 4741 ccaacatcca ataaatcata caggcaaggc aaagaattag caaaattaag caataaagcc 4801 tcagagcata aagctaaatc ggttgtacca aaaacattat gaccctgtaa tacttttgcg 4861 ggagaagcct ttatttcaac gcaaggataa aaatttttag aaccctcata tattttaaat 4921 gcaatgcctg agtaatgtgt aggtaaagat tcaaacgggt gagaaaggcc ggagacagtc 4981 aaatcaccat caatatgata ttcaaccgtt ctagctgata aattcatgcc ggagagggta 5041 gctatttttg agaggtctac aaaggctatc aggtcattgc ctgagagtct ggagcaaaca 5101 agagaatcga tttattttta tatctatcta aaaaaaaact aggaaaaatg aatgtcatca 5161 aatagtattt taacattttt ttttttttta aaaaaaaagt gtcatgacaa aaaaaaaaaa 5221 gtgtcatgac aaaaaaaaaa aaaaaaaaga ggggaaagta attataacta ggttttttta 5281 taatttttac atatttgtta ataactttta attttgaatc atatgatatt acatcgtccc 5341 gttgaaaaaa aaaaaaaaaa tttttttcaa acattttcat tttttaaaaa atgatataaa 5401 attttaaact aaactatttt attaaataca aacatataac tttatcttca atttttttgg 5461 tttatacata tttatgttcg tactgaagta tagatcttat tactaaagtt tcaaaagttt 5521 taaaaaaaat taaaggtaaa tatataactt tctgtttttt tcaattctgt catgacaaaa 5581 aaaaaaaagt gtcatgacaa aaaaaaaaaa aaaaaaaatt ttcttcaata ggtattgaaa 5641 tgacctccgt ttttaataaa aagtatatat ttgtgctcgt ctaggatcta ctagtgtcga 5701 ctagataaaa aaaattttta tttattttta ttattttgaa ttaaatagat tacaaatgat 5761 tacaaattaa ttaatcccat caaatcttta aaaaaaaaaa tggtttaaaa aaacttgggt 5821 tggttaatta ttatttgaaa attttaaaac ccaaattaaa aaaaaaaaat gggattcaaa 5881 aatttttttt tttttttttt tttttttttt tttttttttt ttttttttca gattgcataa 5941 aaagattttt tttttttttt tttcttattt cttaaaacaa ataaattaaa ttaaataaaa 6001 aataaaaatc agatccaagc ttaaaaaatg gatggtaccg agctcatgag ctttttaaga 6061 ccatccattt ggagatcttc tccaatgcaa atggttcaat tgttcgttca aattgaagca 6121 gctcatgaca ctgtcgatga gttgggtaaa ttaggactta ttcaattttt agatgataat 6181 gaacatgtta atttattcca aagaaatttc gtaaatgaag ttaaacgttg tgatgatatg 6241 gaaaagaaat taaaattctt tgaagatcaa gttaaaaagg aaccaaaatt acaaaaactt 6301 ttaccagata atatgttatc agttgttgat gatgattcac aaatggatga attggaaggt 6361 cgtttcgatg aattagaatc tgaattaaaa caagttaatg ccaatcaaga gacactccaa 6421 agaaattaca atgaattaat tcaattacgt catgtattga ccaaggattc agtattcttc 6481 caagagaatc caaatttgat cgaaggtgaa ggtcatgaac atagcgcacg tagtccattg 6541 cttgcagagg atcttcacgt ttcagaagtt gccaaacaag gtgttaaatt aggtttcatc 6601 actggtgtta tgaatacaga caagatgcca caattccaac gttcactttg gagaaccacc 6661 cgtggtaaca attatgtcaa ggatgcacgt atcgaagagg aaatcattga tccacaaact 6721 ggcgaggaaa ctgccaagac tgtattcatt gttttcttcc aaggtgagag attacaacaa 6781 aaaattaaaa agatttgtga atcatttggt gccaacatct acgattgtcc agataattca 6841 ttcgaacgtt caaatctttt acaaaaagtt actgttcgta tcactgatct ctatgaagtt 6901 ttacaacgtt caaaggatca caagagacaa actttagcag gtatcgtacc aagactttac 6961 tcatggaaga agaaggtttt actcgagaaa tcaatctatc acactatgaa tcttttcgat 7021 tatgatgttg gtagaaaatg tttaatcgcc aaaggttgga caccaaaaga taagatcgaa 7081 gagattcaat tggctcttcg tacagctacc acccgtagtg gtgctttggt accatcagtt 7141 ttatcaatca taaagactga aggttcacca ccaactcatt tcgaaactaa taaatacaca 7201 agttcattcc aagagattgt caatgcctat ggtattgcac attatcgtga ggttaatcca 7261 gccgttttaa ccatcgttac tttcccattc cttttcggtg ttatgtttgg tgatgttggt 7321 catggtgctc tcctcttact cagtgccctc ggtttaatct ctttggaaaa gaaattggcc 7381 ggtaagaaat tgaatgaatt aattcaaatg cctttcgatg gtcgttatgt actcttcctt 7441 atgtctctct tctccattta tgttggtttc atttacaatg aatgtttctc aattccaatg 7501 aatattttcg gttctcaata taaccttaat tcaaccaccg gtctttacac ttatcaacat 7561 accgatcgtg tttatccagt tggtgtcgat ccactctgga aaggtgctcc aaatgaattg 7621 gtctactata acagtttcaa aatgaagtta tccattatct ttggtgtcgt tcaaatgagt 7681 gttggtattt gtttctcatt gttgaattac ttaaatcaaa aaggtccaat caagattgta 7741 aacattctca ctcaattcgt tccacaaatg attttccttt ggtctatctt tggttatatg 7801 tctgtcttga tcatcttaaa atgggtcgtt ccatatcgtt catttgaggt tgacaaagtt 7861 gatccaccat tcattcttcc aaccattatc gctatgttct tgtcaccagg tggtacacca 7921 gatgttgtct tcttctctgg tcaaggtgct gttcaaaccg ctctcttatt cttggctctc 7981 atttccattc cagttatgtt ggtcattaaa ccattgttca tgaagagatt ccacttccaa 8041 gaagtcgaaa gaaagaaatt aggtcaccac gaagaggaac atgatgatga agctctctac 8101 actggtcatc acggtgaaga atttgaaatg ggtgaagtct ttgttcatca agtcattcat 8161 accatcgagt ttgttttggg tgctgtttca aatactgcct cttatcttcg tttatgggct 8221 ctttcattgg ctcactctga gttatcttct gtattttggg aacgtattct cattggtcaa 8281 gtcgaacgtg gtaatccatt cttggctttc gttggttttg gtgcttggtt aggtgcctct 8341 gttgctgtac ttttacttat ggaatccctt tctgctttct tgcacgctct tagacttcat 8401 tgggtagaat tccaaaataa attctatatt ggtgatggtg ttagattcat tccatacagt 8461 gccactcgta ttctttctga agatgaactg cagggtgcag gtgcaggtgc aggtgcaggt 8521 gcaatgagta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat 8581 ggcgatgtta atgggcaaaa attctctgtc agtggagagg gtgaaggtga tgcaacatac 8641 ggaaaactta cccttaaatt tatttgcact actgggaagc tacctgttcc atggccaaca 8701 cttgtcacta ctttcactta tggtgttcaa tgcttttcaa gatacccaga tcatatgaaa 8761 cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt 8821 tacaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt 8881 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac 8941 aaaatggaat acaactataa ctcacataat gtatacatca tggcagacaa accaaagaat 9001 ggaatcaaag ttaacttcaa aattagacac aacattaaag atggaagcgt tcaattagca 9061 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat 9121 tacctgtcca cacaatctgc cctttccaaa gatcccaacg aaaagagaga tcacatgatc 9181 cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa 9241 tctagaggat c //