Publications
Vorozhko VV, Emanuele MJ, Kallio MJ, Stukenberg PT, Gorbsky GJ.
Multiple mechanisms of chromosome movement in vertebrate cells mediated through the Ndc80 complex and dynein/dynactin.
Chromosoma. 2008 Apr;117(2):169-79. Epub 2007 Dec 4. [PubMed]
[Full Text]
[Supplemental Material]
[Videos]
Pouwels J, Kukkonen AM, Lan W, Daum JR, Gorbsky GJ, Stukenberg T, Kallio MJ.
Shugoshin 1 plays a central role in kinetochore assembly and is required for kinetochore targeting of Plk1.
Cell Cycle. 2007 Jul;6(13):1579-85. Epub 2007 May 16. [PubMed]
Eyster CA, Duggins QS, Gorbsky GJ, Olson AL.
Microtubule network is required for insulin signaling through activation of Akt/protein kinase B: Evidence that insulin stimulates vesicle docking/fusion but not intracellular mobility.
J Biol Chem. 2006 Dec 22;281(51):39719-27. Epub 2006 Oct 26. [PubMed]
[Full Text] [Supplemental Material]
[Videos]
Potapova TA, Daum JR, Pittman BD, Hudson JR, Jones TN, Satinover DL, Stukenberg PT, Gorbsky GJ.
The reversibility of mitotic exit in vertebrate cells.
Nature. 2006 Apr 13;440(7086):954-8. [PubMed]
[Full Text] [Supplemental Material]
Yamada HY, Gorbsky GJ.
Cell-based expression cloning for identification of polypeptides that hypersensitize mammalian cells to mitotic arrest.
Biological Procedures Online. 2006 Apr 10;8(1):36-43. [Abstract] [Full Text]
Yamada HY, Gorbsky GJ.
Tumor suppressor candidate TSSC5 is regulated by UbcH6 and a novel ubiquitin ligase RING105.
Oncogene. 2006 Mar 2;25(9):1330-9.
[PubMed]
Daum JR, Gorbsky GJ.
Lysed cell models and isolated chromosomes for the study of kinetochore/centromere biochemistry in vitro.
Methods. 2006 Jan;38(1):52-9.
[PubMed]
Yamada HY, Gorbsky GJ.
Inhibition of TRIP1/S8/hSug1, a component of the human 19S proteasome, enhances mitotic apoptosis induced by spindle poisons.
Mol Cancer Ther. 2006 Jan;5(1):29-38.
[PubMed]
Ahonen LJ, Kallio MJ, Daum JR, Bolton M, Manke IA, Yaffe MB, Stukenberg PT, Gorbsky GJ.
Polo-like kinase 1 creates the tension-sensing 3F3/2 phosphoepitope and modulates the association of spindle-checkpoint proteins at kinetochores.
Curr Biol. 2005 Jun 21;15(12):1078-89.
[PubMed]
Beardmore VA, Ahonen LJ, Gorbsky GJ, Kallio MJ.
Survivin dynamics increases at centromeres during G2/M phase transition and is regulated by microtubule-attachment and Aurora B kinase activity.
J Cell Sci. 2004 Aug 15;117(Pt 18):4033-42. Epub 2004 Jul 27.
[PubMed]
[Full Text]
[Supplemental Material]
[Videos]
Rundle DR, Gorbsky G, Tsiokas L.
PKD2 interacts and co-localizes with mDia1 to mitotic spindles of dividing cells: role of mDia1 IN PKD2 localization to mitotic spindles.
J Biol Chem. 2004 Jul 9;279(28):29728-39. Epub 2004 Apr 28.
[PubMed]
[Full Text]
Gorbsky GJ.
Mitosis: MCAK under the aura of Aurora B.
Curr Biol. 2004 May 4;14(9):R346-8. Review.
[PubMed]
McCleland ML, Kallio MJ, Barrett-Wilt GA, Kestner CA, Shabanowitz J, Hunt DF, Gorbsky GJ, Stukenberg PT.
The vertebrate Ndc80 complex contains Spc24 and Spc25 homologs, which are required to establish and maintain kinetochore-microtubule attachment.
Curr Biol. 2004 Jan 20;14(2):131-7.
[PubMed]
McCleland ML, Gardner RD, Kallio MJ, Daum JR, Gorbsky GJ, Burke DJ,
Stukenberg PT.
The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity.
Genes Dev. 2003 Jan 1;17(1):101-14.
[PubMed]
[Full Text]
[Supplemental Material]
Kallio MJ, Beardmore VA, Weinstein J, Gorbsky GJ.
Rapid microtubule-independent dynamics of Cdc20 at kinetochores and centrosomes in mammalian cells.
J Cell Biol. 2002 Sep 2;158(5):841-7. Epub 2002 Aug 26.
[PubMed]
[Full Text]
[Supplemental Material]
Tavormina PA, Come MG, Hudson JR, Mo YY, Beck WT, Gorbsky GJ.
Rapid exchange of mammalian topoisomerase II alpha at kinetochores and chromosome arms in mitosis.
J Cell Biol. 2002 Jul 8;158(1):23-9. Epub 2002 Jul 8.
[PubMed]
[Full Text]
[Supplemental Material]
Topper LM, Campbell MS, Tugendreich S, Daum JR, Burke DJ, Hieter P, Gorbsky GJ.
The dephosphorylated form of the anaphase-promoting complex protein Cdc27/Apc3 concentrates on kinetochores and chromosome arms in mitosis.
Cell Cycle. 2002 Jul-Aug;1(4):282-92.
[PubMed]
Null AP, Hudson J, Gorbsky GJ.
Both alpha and beta isoforms of mammalian DNA topoisomerase II associate with chromosomes in mitosis.
Cell Growth Differ. 2002 Jul;13(7):325-33.
[PubMed]
[Full Text]
Messenger MM, Saulnier RB, Gilchrist AD, Diamond P, Gorbsky GJ, Litchfield DW.
Interactions between protein kinase CK2 and Pin1. Evidence for phosphorylation-dependent interactions.
J Biol Chem. 2002 Jun 21;277(25):23054-64. Epub 2002 Apr 8.
[PubMed]
[Full Text]
Kallio MJ, McCleland ML, Stukenberg PT, Gorbsky GJ.
Inhibition of aurora B kinase blocks chromosome segregation, overrides the spindle checkpoint, and perturbs microtubule dynamics in mitosis.
Curr Biol. 2002 Jun 4;12(11):900-5.
[PubMed]
Giodini A, Kallio MJ, Wall NR, Gorbsky GJ, Tognin S, Marchisio PC, Symons
M, Altieri DC.
Regulation of microtubule stability and mitotic progression by survivin.
Cancer Res. 2002 May 1;62(9):2462-7.
[PubMed]
[Full Text]
Gorbsky GJ.
The mitotic spindle checkpoint.
Curr Biol. 2001 Dec 11;11(24):R1001-4. [PubMed]
Escargueil AE, Plisov SY, Skladanowski A, Borgne A, Meijer L, Gorbsky GJ, Larsen AK.
Recruitment of cdc2 kinase by DNA topoisomerase II is coupled to chromatin remodeling.
FASEB J. 2001 Oct;15(12):2288-90. Epub 2001 Aug 17.
[PubMed]
[Full Text]
Topper LM, Bastians H, Ruderman JV, Gorbsky GJ.
Elevating the level of Cdc34/Ubc3 ubiquitin-conjugating enzyme in mitosis inhibits association of CENP-E with kinetochores and blocks the metaphase
alignment of chromosomes.
J Cell Biol. 2001 Aug 20;154(4):707-17. Erratum in: J Cell Biol 2002 Jul
22;158(2):371.
[PubMed]
[Full Text]
Daum JR, Tugendreich S, Topper LM, Jorgensen PM, Hoog C, Hieter P, Gorbsky
GJ.
The 3F3/2 anti-phosphoepitope antibody binds the mitotically phosphorylated anaphase-promoting complex/cyclosome.
Curr Biol. 2000 Nov 30;10(23):R850-2. [PubMed]
Kallio M, Eriksson JE, Gorbsky GJ.
Differences in spindle association of the mitotic checkpoint protein Mad2 in mammalian spermatogenesis and oogenesis.
Dev Biol. 2000 Sep 1;225(1):112-23.
[PubMed]
Campbell MS, Daum JR, Gersch MS, Nicklas RB, Gorbsky GJ.
Kinetochore "memory" of spindle checkpoint signaling in lysed mitotic cells.
Cell Motil Cytoskeleton. 2000 Jun;46(2):146-56.
[PubMed]
Gorbsky GJ, Kallio M, Daum JR, Topper LM.
Protein dynamics at the kinetochore: cell cycle regulation of the metaphase to anaphase transition.
FASEB J. 1999 Dec;13 Suppl 2:S231-4.
[PubMed]
[Full Text]
Bastians H, Topper LM, Gorbsky GL, Ruderman JV.
Cell cycle-regulated proteolysis of mitotic target proteins.
Mol Biol Cell. 1999 Nov;10(11):3927-41.
[PubMed]
[Full Text]
Waters JC, Chen RH, Murray AW, Gorbsky GJ, Salmon ED, Nicklas RB.
Mad2 binding by phosphorylated kinetochores links error detection and checkpoint action in mitosis.
Curr Biol. 1999 Jun 17;9(12):649-52.
[PubMed]
Daum JR, Gorbsky GJ.
Casein kinase II catalyzes a mitotic phosphorylation on threonine 1342 of human DNA topoisomerase IIalpha, which is recognized by the 3F3/2 phosphoepitope
antibody.
J Biol Chem. 1998 Nov 13;273(46):30622-9.
[PubMed]
[Full Text]
Nicklas RB, Campbell MS, Ward SC, Gorbsky GJ.
Tension-sensitive kinetochore phosphorylation in vitro. J Cell Sci. 1998 Nov;111 ( Pt 21):3189-96.
[PubMed]
[Full Text]
Zecevic M, Catling AD, Eblen ST, Renzi L, Hittle JC, Yen TJ, Gorbsky GJ,
Weber MJ.
Active MAP kinase in mitosis: localization at kinetochores and association with the motor protein CENP-E.
J Cell Biol. 1998 Sep 21;142(6):1547-58.
[PubMed]
[Full Text]
Kallio M, Weinstein J, Daum JR, Burke DJ, Gorbsky GJ.
Mammalian p55CDC mediates association of the spindle checkpoint protein Mad2 with the cyclosome/anaphase-promoting complex, and is involved in regulating
anaphase onset and late mitotic events.
J Cell Biol. 1998 Jun 15;141(6):1393-406.
[PubMed]
[Full Text]
Gorbsky GJ, Chen RH, Murray AW.
Microinjection of antibody to Mad2 protein into mammalian cells in mitosis induces premature anaphase.
J Cell Biol. 1998 Jun 1;141(5):1193-205.
[PubMed]
[Full Text]
Renzi L, Gersch MS, Campbell MS, Wu L, Osmani SA, Gorbsky GJ.
MPM-2 antibody-reactive phosphorylations can be created in detergent-extracted cells by kinetochore-bound and soluble kinases.
J Cell Sci. 1997 Sep;110 ( Pt 17):2013-25.
[PubMed]
[Full Text]
Bousbaa H, Correia L, Gorbsky GJ, Sunkel CE.
Mitotic phosphoepitopes are expressed in Kc cells, neuroblasts and isolated chromosomes of Drosophila melanogaster.
J Cell Sci. 1997 Sep;110 ( Pt 17):1979-88.
[PubMed]
[Full Text]
Gorbsky GJ.
Cell cycle checkpoints: arresting progress in mitosis.
Bioessays. 1997 Mar;19(3):193-7. Review.
[PubMed]
Campbell MS, Lovell MA, Gorbsky GJ.
Stability of nuclear segments in human neutrophils and evidence against a role for microfilaments or microtubules in their genesis during differentiation of
HL60 myelocytes.
J Leukoc Biol. 1995 Dec;58(6):659-66.
[PubMed]
Taagepera S, Campbell MS, Gorbsky GJ.
Cell-cycle-regulated localization of tyrosine and threonine phosphoepitopes at the kinetochores of mitotic chromosomes.
Exp Cell Res. 1995 Nov;221(1):249-60.
[PubMed]
Nicklas RB, Ward SC, Gorbsky GJ.
Kinetochore chemistry is sensitive to tension and may link mitotic forces to a cell cycle checkpoint.
J Cell Biol. 1995 Aug;130(4):929-39.
[PubMed]
[Full Text]
Campbell MS, Gorbsky GJ.
Microinjection of mitotic cells with the 3F3/2 anti-phosphoepitope antibody delays the onset of anaphase.
J Cell Biol. 1995 Jun;129(5):1195-204.
[PubMed]
[Full Text]
Gorbsky GJ.
Kinetochores, microtubules and the metaphase checkpoint.
Trends Cell Biol. 1995 Apr;5(4):143-8.
[PubMed]
Taagepera S, Dent P, Her JH, Sturgill TW, Gorbsky GJ.
The MPM-2 antibody inhibits mitogen-activated protein kinase activity by binding to an epitope containing phosphothreonine-183.
Mol Biol Cell. 1994 Nov;5(11):1243-51.
[PubMed]
[Full Text]
Gorbsky GJ.
Cell cycle progression and chromosome segregation in mammalian cells cultured in the presence of the topoisomerase II inhibitors ICRF-187
[(+)-1,2-bis(3,5-dioxopiperazinyl-1-yl)propane; ADR-529] and ICRF-159
(Razoxane).
Cancer Res. 1994 Feb 15;54(4):1042-8.
[PubMed]
Taagepera S, Rao PN, Drake FH, Gorbsky GJ.
DNA topoisomerase II alpha is the major chromosome protein recognized by the mitotic phosphoprotein antibody MPM-2.
Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8407-11.
[PubMed]
[Full Text]
Gorbsky GJ, Ricketts WA.
Differential expression of a phosphoepitope at the kinetochores of moving chromosomes.
J Cell Biol. 1993 Sep;122(6):1311-21.
[PubMed]
[Full Text]
Gorbsky GJ.
Chromosome motion in mitosis.
Bioessays. 1992 Feb;14(2):73-80. Review.
[PubMed]
Gorbsky GJ, Simerly C, Schatten G, Borisy GG.
Microtubules in the metaphase-arrested mouse oocyte turn over rapidly.
Proc Natl Acad Sci U S A. 1990 Aug;87(16):6049-53.
[PubMed]
[Full Text]
Gorbsky GJ, Borisy GG.
Microtubules of the kinetochore fiber turn over in metaphase but not in anaphase.
J Cell Biol. 1989 Aug;109(2):653-62.
[PubMed]
[Full Text]
Gorbsky GJ, Borisy GG.
Hapten-mediated immunocytochemistry: the use of fluorescent and nonfluorescent haptens for the study of cytoskeletal dynamics in living cells.
Methods Cell Biol. 1989;29:175-93. Review. [PubMed]
Gorbsky GJ, Borisy GG.
Microtubule dynamics and the movement of chromosomes.
Prog Clin Biol Res. 1989;318:159-69. [PubMed]
Gorbsky GJ, Sammak PJ, Borisy GG.
Microtubule dynamics and chromosome motion visualized in living anaphase cells.
J Cell Biol. 1988 Apr;106(4):1185-92.
[PubMed]
[Full Text]
Sammak PJ, Gorbsky GJ, Borisy GG.
Microtubule dynamics in vivo: a test of mechanisms of turnover.
J Cell Biol. 1987 Mar;104(3):395-405.
[PubMed]
[Full Text]
Gorbsky GJ, Sammak PJ, Borisy GG.
Chromosomes move poleward in anaphase along stationary microtubules that coordinately disassemble from their kinetochore ends.
J Cell Biol. 1987 Jan;104(1):9-18.
[PubMed]
[Full Text]